Fig. 4

Scanning electron microscopy (SEM) images of the surface of the contact lenses and attachedAcanthamoeba (arrows). While (A), (B), and (C) show SEM images before Acanthamoeba inoculation(original magnification×1,000), (D), (E), and (F) show images after Acanthamoeba inoculation (originalmagnification×100). The surfaces of the cosmetic CLs and nephrite-embedded CLs were rougher comparedwith that of clear CLs. More Acanthamoeba were attached to cosmetic CLs than clear and nephrite-embeddedCLs. (F) Acanthamoeba trophozoites on the nephrite-embedded CL surface exhibit reduced acanthopodiaformation, suggesting encystment-like or stress-associated morphological changes.