Fig. 4
From: LncRNA FTX promotes myocardial fibrosis by sponging miR-335-3p to regulate TFEC/ILK signaling
The regulation of TFEC/ILK axis on the Akt/GSK3βsignaling pathway in cardiac fibroblasts and the regulation of miR-335-3p on myocardial fibrosis and TFEC. (A) CFs were transfected with TGF-β1, si-ILK, si- TFEC or si-NC and then stimulated with TGF-β1. Protein levels of p-Akt (Ser473), total Akt, p-GSK3β (Ser9), and total GSK3β were analyzed by Western blotting. (B) Multiple miRNAs, including miR-374-5p, miR-5096, miR-335-3p, miR-769-5p, miR-873-5p, and miR-506-5p, were predicted to have binding sites with the transcription factor TFEC. The expression levels of these miRNAs were examined. The level of miR-335-3p was significantly downregulated in the Myocardial Fibrosis tissue compared to Control. (C) CFs were transfected with a miR-335-3p inhibitor to reduce miR-335-3p levels, and protein expression of RUNX1 and TFEC was analyzed by western blotting. **p < 0.01 vs. indicated groups.
