Fig. 6
From: LncRNA FTX promotes myocardial fibrosis by sponging miR-335-3p to regulate TFEC/ILK signaling
TFEC is a target of miR-335-3p. (A,B) CFs were transfected with either miR-335-3p mimic or miR-335-3p inhibitor, and TFEC protein expression was measured by western blotting and quantified. It shows that miR-335-3p negatively regulates TFEC expression. (C) The predicted binding sequences between miR-335-3p and TFEC and the mutant sites of TFEC. (D,E) Luciferase reporter assays confirmed the interaction between miR-335-3p and TFEC. In the TFEC-wild-type group, the miR-335-3p mimic significantly reduced luciferase activity, while the inhibitor increased it; no activity changes were observed in the TFEC-mutant group, indicating that miR-335-3p regulates TFEC expression by direct targeting.*p < 0.05 and **p < 0.01 vs. indicated groups.
