Abstract
Motor impairment following stroke frequently leads to long-term disability, limiting independence and quality of life. Brain–Computer Interface (BCI) systems integrating motor imagery (MI) with virtual reality (VR) offer promising avenues for enhancing neuroplasticity and engagement through immersive, real-time, and proprioceptive feedback. Yet, identifying reliable electroencephalography (EEG)-based biomarkers that reflect or predict recovery remains challenging. This study investigated the relationship between event-related desynchronization (ERD) dynamics during MI–VR training and motor recovery in individuals with chronic stroke. Fourteen participants with stroke (9 experimental, 5 control) completed a 4-week VR–BCI intervention and were compared with a non-stroke reference cohort (N = 35). Linear mixed-effects models assessed ERD modulation across sessions and groups, and a two-stage regression evaluated the predictive value of ERD features for Fugl–Meyer Assessment (FMA) gains. Results showed no significant ERD change across sessions, but stroke participants exhibited significantly reduced ERD compared to controls. Baseline ERD amplitude predicted motor improvement, whereas ERD progression did not. Ipsilateral ERD showed a compensatory trend in ischemic stroke. These findings indicate that baseline ERD may serve as a stronger prognostic biomarker than short-term ERD dynamics, supporting the development of personalized VR–BCI rehabilitation strategies for chronic stroke recovery.
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Data availability
The data that support the findings of this study are available from the corresponding author, upon reasonable request.
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Acknowledgements
This work is supported by the LARSyS - FCT Project (DOI: 10.54499/LA/P/0083/2020, 10.54499/UIDP/50009/2020, and 10.54499/UIDB/50009/2020), the NeurAugVR (PTDC/CCI-COM/31485/2017), the NOISyS project (DOI: 10.54499/2022.02283.PTDC), the NOVA LINCS (DOI: 10.54499/UIDB/04516/2020 and 10.54499/UIDP/04516/2020) with the financial support of FCT.IP (2021.05646.BD) and the Recovery and Resilience Plan under the application no 761 submitted to the measure Polos de Inovação Digital (DIH) under the terms of AAC no. 03/C16 i03/2022. Finally, we would like to acknowledge Audrey Aldridge, Carolina Jorge, Diego Andres Blanco-Mora, Sofia Ferreira, Mónica Rosa, and Sidonio Fernandes for assisting with the patient’s preparation and data acquisition at the hospital.
Funding
This work is supported financially by FCT through the LARSyS - FCT Project (DOI: 10.54499/LA/P/0083/2020, 10.54499/UIDP/50009/2020, and 10.54499/UIDB/50009/2020), the NeurAugVR (PTDC/CCI-COM/31485/2017), the NOISyS project (DOI: 10.54499/2022.02283.PTDC), the FCT grant: 10.54499/2021.05646.BD, the NOVA LINCS (DOI: 10.54499/UIDB/04516/2020 and 10.54499/UIDP/04516/2020) with the financial support of FCT.IP (2021.05646.BD) and the Recovery and Resilience Plan under the application no 761 submitted to the measure Polos de Inovação Digital (DIH) under the terms of AAC no. 03/C16 i03/2022.
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CRediT taxonomy: MV: Data curation; Formal analysis; Investigation; Visualization; Writing - original draft DB: Data curation; Investigation; Validation; Writing - review & editing. JC-F: Data curation; Investigation; Validation; Writing - review & editing. SBB: Conceptualization; Investigation; Funding acquisition; Project Administration; Resources; Writing - review & editing. PF: Conceptualization; Investigation; Funding acquisition; Resources; Supervision; Validation; Writing - review & editing. AV: Conceptualization; Investigation; Software; Methodology; Supervision; Resources; Validation; Writing - review & editing.
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Valente, M., Branco, D., Bermúdez i Badia, S. et al. EEG-based predictors of motor recovery during immersive VR-BCI rehabilitation. Sci Rep (2026). https://doi.org/10.1038/s41598-026-39106-1
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DOI: https://doi.org/10.1038/s41598-026-39106-1


