Fig. 4
From: Metapanax delavayi extract as a neurocutaneous modulator via CRHR1/POMC/MC1R signaling
MDE suppressed substance P-induced release of inflammatory factors in HaCaT cells. (A) CCK-8 assay, HaCaT cells were supplemented with 15, 30, 60, 120, 240, 480 μg/mL MDE for 24 h (N = 3). (B-D) RT-qPCR was used to determine the mRNA expression levels of proinflammatory cytokines (IL-6, IL-8, and IL-1α) (N = 4). (E-G) The content of IL-6, IL-8 and IL-1α in different groups (N = 5). Data are presented as mean ± SEM. p value using the one-way ANOVA. * p ≤ 0.05, ** p ≤ 0.01 versus control; # p ≤ 0.05, ## p ≤ 0.01 versus substance P-induced.
