Fig. 3
The lifespan extension of fln-2(ot611) mutants is associated with dietary restriction pathways without affecting their food intake. a–d Lifespan curves of wild-type N2, fln-2(ot611), daf-16(mgDf50) (a), daf-2(e1370) (b), pmk-1(km25) (c), eat-2(ad1113) (d) single mutants, and fln-2(ot611);daf-16(mgDf50) (a), fln-2(ot611);daf-2(e1370) (b), fln-2(ot611);pmk-1(km25) (c), fln-2(ot611);eat-2(ad1113) (d) double mutants. e Summary of mean lifespans. Data are presented as mean lifespan ± SEM. p-Values were determined using the log‐rank test. *p < 0.05; **p < 0.01; ***p < 0.001; and n.s., not significant. See Supplementary Table 7 for details. f Food intake of worms was assessed in liquid medium. g Relative food intake of wild-type N2 and fln-2(ot611) mutants. h, i Pharyngeal pumping frequency of wild-type N2 and fln-2(ot611) mutants at Day 1(h) or Day 5 (i) stages. Data in (g-i) are shown as means ± SEM. Differences between multiple groups were determined using one-way ANOVA with Tukey’s multiple comparison test. ***p < 0.001; and n.s., not significant.
