Fig. 2

Temperature compensation of circadian oscillations under varying KaiB concentrations. (a), KaiC phosphorylation at 35 °C with four KaiB concentrations (1.7 µM, green; 3.4 µM, orange; 6.8 µM, blue; 10.2 µM, red). Closed circles represent measured values, and dotted lines are drawn for visualization. Two additional replicates are provided in the Supplementary Information (Fig. S3). (b), KaiC phosphorylation at 25 °C with the same four KaiB concentrations. Open circles represent measured values, and solid lines are drawn for visualization. Two additional replicates are provided in the Supplementary Information. (c), Circadian periods at each KaiB concentration and temperature (25 °C, open circles; 35 °C, closed circles). The linear correlation at 25 °C is described by y = 21.30 – 0.129x (R² = 0.871, p = 0.07) and at 35 °C by y = 21.11 – 0.101x (R² = 0.551, p = 0.26). Data represent the mean of three independent measurements ± s.e.m. (d), Temperature compensation of circadian oscillations expressed as Q₁₀ values of circadian frequency (n = 3). The linear correlation is described by y = 1.0088 – 0.0016x (R² = 0.136, p = 0.63).