Fig. 3

Temperature compensation of circadian oscillations under varying NaCl concentrations. (a), KaiC phosphorylation at 25 °C with four NaCl concentrations (100 mM, green; 150 mM, orange; 200 mM, blue; 250 mM, red). Open circles represent measured values, with solid lines drawn for visualization. Two additional replicates are provided in the Supplementary Information (Fig. S4). (b), KaiC phosphorylation at 35 °C with the same four NaCl concentrations. Closed circles represent measured values, with dotted lines drawn for visualization. Two additional replicates are provided in the Supplementary Information. (c), Circadian periods at each NaCl concentration and temperature (25 °C, open circles; 35 °C, closed circles). The linear correlation at 25 °C is described by y = 21.88–0.0074x (R² = 0.984, p = 0.01) and at 35 °C by y = 25.24 – 0.0256x (R² = 0.995, p = 0.002). Data represent the mean of three independent measurements ± s.e.m. (d), Temperature compensation of circadian oscillations, expressed as Q₁₀ values of circadian frequency (n = 3). The linear correlation is described by y = 1.158 – 0.00083x (R² = 0.992, p = 0.004).