Fig. 2

Effect of pramipexole on Nrf2/SLC7A11/GPX4 proteins and genes in cells subjected to OGD/R. (a–e) Protein expression of Nrf2, SLC7A11, GPX4, p53 in HT22 cells after OGD/R. (f–i) Gene expression of Nrf2, SLC7A11, GPX4, p53 in HT22 cells after OGD/R. (j–o) Immunofluorescence staining and detection of Nrf2, SLC7A11, GPX4 in HT22 cells after OGD/R. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.