Table 3 Optimised IAA yield from different factors using DOE.
Run | Rotation speed (RPM) | Time (No. of Days) | Tryptophan | Glucose | Intracellular Yield of IAA mg/ml | Extracellular Yield of IAA mg/ml |
|---|---|---|---|---|---|---|
1 | −1 | −1 | −1 | −1 | 0.02 | 0.02 |
2 | −1 | + 1 | −1 | −1 | 0.03 | 0.089 |
3 | −1 | −1 | + 1 | −1 | 0.25 | 0.67 |
4 | −1 | + 1 | + 1 | −1 | 0.35 | 1.63 |
5 | −1 | −1 | −1 | + 1 | 0.17 | 0.71 |
6 | −1 | + 1 | −1 | + 1 | 0.12 | 1.31 |
7 | −1 | −1 | + 1 | + 1 | 0.39 | 2.20 |
8 | −1 | + 1 | + 1 | + 1 | 0.59 | 2.25 |
9 | + 1 | −1 | −1 | −1 | 0.08 | 1.51 |
10 | + 1 | + 1 | −1 | −1 | 0.02 | 0.55 |
11 | + 1 | −1 | + 1 | −1 | 0.48 | 0.94 |
12 | + 1 | + 1 | + 1 | −1 | 0.26 | 0.94 |
13 | + 1 | −1 | −1 | + 1 | 0.25 | 0.06 |
14 | + 1 | + 1 | −1 | + 1 | 0.10 | 1.21 |
15 | + 1 | −1 | + 1 | + 1 | 0.63 | 0.23 |
16 | + 1 | + 1 | + 1 | + 1 | 0.37 | 1.84 |
