Fig. 2

3-D structure modeling and validation of the chimeric multi-epitope vaccine. (A) Chosen epitopes and linkers used in the chimeric subunit vaccine design, (B) Ramachandran plot analysis showing the distribution of backbone dihedral angles, indicating model stereochemical quality. (C) Predicted 3-D structure of the proposed chimeric multiepitope vaccine, (D) Z-score analysis from ProSA-web, representing the overall model quality compared to experimentally determined structures.