Fig. 5

Normal mode analysis (NMA) of the chimeric multi-epitope vaccine construct in complex with TLR-4. (A) Deformability plot showing flexible regions within the complex. (B) B-factor plot comparing experimental and predicted residue fluctuations. (C) Eigenvalue representing the overall structural stiffness. (D) Variance plot showing individual and cumulative variances of the modes. (E) The covariance matrix of residue movements indicates correlated (red), uncorrelated (white), and anti-correlated (blue) motions. (F) Elastic network model illustrating inter-residue connections as springs. (G) Summary plot of the overall mobility and interaction dynamics derived from iMODS.