Fig. 6

In silico cloning of the codon-optimized chimeric vaccine into the pET-28a(+) vector. The diagram shows the successful insertion of the gene using Sca I and Bmt I sites, confirming it can be expressed in E. coli.

In silico cloning of the codon-optimized chimeric vaccine into the pET-28a(+) vector. The diagram shows the successful insertion of the gene using Sca I and Bmt I sites, confirming it can be expressed in E. coli.