Table 1 The sequence of oligonucleotide primers.
Primers’ name | Sequence (5’−3’) | Annealing (℃) | Product size (bp) | Reference |
|---|---|---|---|---|
P. aeruginosa identification | ||||
16 S rRNA-F 16 S rRNA-R | GGGGGATCTTCG GACCTCA TCCTTAGAGTGCCCACCCG | 58 | 965 | |
Carbapenemase genes | ||||
blaOXA-48-F blaOXA-48-R | GCGTGGTTAAGGATGAACAC CATCAAGTTCAACCCAACCG | 52 | 438 | |
blaIMP-F blaIMP-R | GGAATAGAGTGGCTTAAYTCTC GGTTTAAYAAAACAACCACC | 232 | ||
blaVIM-F blaVIM-R | GATGGTGTTTGGTCGCATA CGAATGCGCAGCACCAG | 390 | ||
blaKPC-F blaKPC-R | CGTCTAGTTCTGCTGTCTTG CTTGTCATCCTTGTTAGGCG | 798 | ||
blaSIM-F blaSIM-R | AAAATCTGGGTACGCAAACG ACATTATCCGCTGGAACAGG | 570 | ||
