Fig. 3
Cytokine expression in BV2 microglia following 1 h OGD and 24 h reoxygenation (OGD/R) ± VitC (5 µg/mL). (A). RT-qPCR(\(\:\stackrel{-}{X}\)±SD): OGD/R up-regulated COX-2, IL-1β, IL-6, TNF-α, iNOS, CD206 and IL-10 mRNA (P<0.05 vs. CON). VitC reversed the increase in COX-2, IL-1β, IL-6, iNOS and TNF-α and further elevated IL-10 and CD206 (P < 0.05 vs. OGD/R). (B). ELISA\(\:\stackrel{-}{X}\)±SD: OGD/R increased, and VitC reduced IL-1β, IL-6, TNF-α while increasing IL-10 protein levels with ELISA on conditioned media from BV2 monocultures (P < 0.05). RT-qPCR data are from n = 3 independent biological replicates (BV2 cells from 3 independent passages), with triplicate technical replicates each. ELISA data are from n = 3 independent biological replicates, each measured in duplicate.
