Fig. 4

OPCs apoptosis and microglial cytokine expressions in OPC/BV2 co-culture. (A). Apoptosis(\(\:\stackrel{-}{X}\)±SD)(50 μm): OGD/R increased TUNEL⁺/PDGFRα⁺ OPCs (P<0.001 vs. CON); VitC reduced apoptosis (P < 0.01 vs. OGD/R). (B). Cytokine expression (RT-qPCR) \(\:\stackrel{-}{X}\)±SD: OGD/R up-regulated COX-2, IL-1β, IL-6, TNF-α, iNOS, CD206 and IL-10 mRNA (P < 0.05 vs. CON). VitC reversed the increase in COX-2, IL-1β, IL-6, iNOS and TNF-α and further elevated IL-10 and CD206 (P<0.05 vs. OGD/R). (C). Cytokine expression (ELISA) \(\:\stackrel{-}{X}\)±SD: OGD/R increased, and VitC reduced IL-1β, IL-6, TNF-α while increasing IL-10 protein levels with ELISA on conditioned media collected from OPC-BV2 co-cultures at the end of the 24 h reoxygenation period (P < 0.05). Scale bar=50 μm and located in the bottom-right corner. Apoptosis data are from n = 3 independent biological replicates with ≥ 5 fields quantified per replicate; qPCR and ELISA data are from n = 3 independent biological replicates with triplicate and duplicate technical replicates, respectively.