Fig. 2

Mice carrying the Tns1 floxed alleles exhibit normal reproductive fitness and subsequent conditional TNS1 knockout in adult mice does not impact body weight. Number of litters per mating pair (A) and number of pups per litter (B) were analyzed in n = 5 breeding pairs in indicated colonies. The comparison includes three groups of TNS1-expressing mice: in-house bred WT mice (C57BL6/J), mice carrying the Tns1 floxed alleles on C57BL6/J background without a Cre-driver (B6.Tns1^{lox, lox}) and with a Cre-driver (Rosa^Cre/+; Tns1^{lox, lox}). One-way ANOVA was utilized for statistical assessments. (C) Rosa^Cre/+;Tns1^lox/lox (Tns1-/-) and TNS1 expressing controls (Tns1+/+) weights were collected for 14 days thereafter (n = 14 for Tns1+/+ and 7 for Tns1 -/-). Two-way ANOVA was used for statistical assessments. p < 0.05 was considered statistically significant and was not met in any of the comparisons. Data is presented as mean ± SD.