Fig. 5
From: Development and characterization of an inducible Tensin1 deficient transgenic murine model
RNA sequencing analysis reveals differential expression of genes involved in immune system regulation and cytoskeletal organization. (A) KEGG Pathway Enrichment Analysis of the top eight differentially expressed pathways shows a number of immune system regulated pathways in tamoxifen-treated RosaCre/+;Tns1lox/lox (Tns1-/-) mice compared to TNS1 expressing controls (Tns1+/+). Bubble size indicates the number of genes identified in each pathway. (B) Volcano plot depicting differentially expressed genes in lung lysates from Tns1-/- and Tns1+/+ control mice. The colored points depict differentially expressed genes (adjusted p < 0.05) with a -log(FDR) >1.303. The negative (blue) and positive (red) log10(FC) indicate downregulated genes such as Nfkbia, Trem1, Bhlhe40 and Clec4e and upregulated genes such as Spib and Ms4a1, respectively, in Tns1-/- samples. C. Heatmap showing Z-scores for normalized expression of the top 50 differentially expressed genes with an adjusted p value <0.05. (n = 3 for Tns1 -/- and 5 for Tns1 +/+) and illustrates upregulation of Cd19, Cd22, Spib, and Bank1 and downregulation of Trem1, Treml4, Plaur, Cd300e, Acod1, and Clec4e in Tns1-/- mice compared to Tns1+/+.
