Fig. 7
From: Development and characterization of an inducible Tensin1 deficient transgenic murine model
TNS1 knockout reveals upregulation of B cell genes and a trend toward increased lymphocyte presence in the bronchoalveolar lavage fluid. (A) STRING: functional protein association networks analysis with supervised MCL clustering (inflation parameter of 2.2) of differentially regulated genes (adjusted p < 0.05) revealed differential expression in the myeloid cell cluster (n = 0 downregulated and 16 upregulated genes) in tamoxifen-treated RosaCre/+;Tns1lox/lox (Tns1-/-) mice compared to TNS1 expressing controls (Tns1+/+). Darker and wider gene outlines indicate a greater level of differential gene expression, while weight and color of the lines connecting the nodes are STRING features that indicate the strength of the prediction of the interaction. (B, C) Mouse lungs were collected for reverse-transcription quantitative polymerase chain reaction analysis of B cell markers, Spib (B) and Cd19 (C). (D–G) Mouse bronchoalveolar lavage was performed and BAL fluid was collected for analysis of macrophages (D), neutrophils (E), lymphocytes (F), and eosinophils (G). Unpaired Student’s t-test was utilized for statistical analyses directly or on log-transformed data that did not pass normality testing (*p < 0.05). Data is presented as mean ± SD.
