Table 1 Primer information in the study.
# | Gene name (primer name) | Usage | Amplified region (UCSC hg38) | PCR primers | Product |
|---|---|---|---|---|---|
size (bp) | |||||
1 | HOXC8 | real-time RT-PCR | chr12: 54,009,650 − 54,011,212 | F: TAGCGAAGGACAAGGCCACT | 195 |
R: CCCAGGGCATGAGAGACTTC | |||||
2 | TGFB1 | real-time RT-PCR | chr19:41348370–41,352,746 | F: AGGCCGACTACTACGCCAAG | 143 |
R: GGGTTCAGGTACCGCTTCTC | |||||
3 | COL1A1 | real-time RT-PCR | chr17: 50,184,101 − 50,201,631 | F: AGAGTCTACATGTCTAGGGTCTAGA | 167 |
R: GTACCTGAGGCCGTTCTGTAC | |||||
4 | COL1A2 | real-time RT-PCR | chr7: 94,394,895 − 94,431,227 | F: TGAAGATGGTCACCCTGGAAAAC | 450 |
R: GCTGTCCCTTCAATCCATCCA | |||||
5 | MMP1 | real-time RT-PCR | chr11: 102,789,919 − 102,798,160 | F: GATGTGGCTCAGTTTGTCCTCA | 290 |
R: CTTGGTGAATGTCAGAGGTGTGA | |||||
6 | MMP2 | real-time RT-PCR | chr16: 55,479,198 − 55,506,691 | F: AACTACAACTTCTTCCCTCGCAAG | 350 |
R: AAGTTGATCATGATGTCTGCCTCTC | |||||
7 | MMP14 | real-time RT-PCR | chr14:22,841,959 − 22,842,456 | F: GAGATCAAGGCCAATGTTCGAAG | 124 |
R: CCTCGTATGTGGCATACTCGC | |||||
8 | TIMP1 | real-time RT-PCR | chrX: 47,582,436 − 47,586,789 | F: GATACTTCCACAGGTCCCACAAC | 240 |
R: CCAACAGTGTAGGTCTTGGTGAAG | |||||
9 | TIMP2 | real-time RT-PCR | chr17: 78,852,977 − 78,925,387 | F: CAGAAGAAGAGCCTGAACCACA | 310 |
R: GTTGATGTTCTTCTCTGTGACCCA | |||||
10 | TIMP3 | real-time RT-PCR | chr22: 32,801,705 − 32,863,041 | F: CCTTAAGCTGGAGGTCAACAAGTA | 400 |
R: CAGGACTTGATCTTGCAGTTACAAC | |||||
11 | GAPDH | real-time RT-PCR | chr12: 6,534,840-6,536,570 | F: AGGTGAAGGTCGGAGTCA | 99 |
R: GGTCATTGATGGCAACAA | |||||
12 | HOXC8 CDS | vector construction | - | F: ATTAAGGATCCTCGAGTAGGCCACCATGAGCTCCTACTTCGTCAA | 763 |
R: GAGGGGCGGATCTTAGTCCTTGTTTTCTTCCT | |||||
13 | IRES2-AcGFP | vector construction | - | F: GAAAACAAGGACTAAGATCCGCCCCTCTCCCTC | 1350 |
Please merge this cell with the cell above. | R: CCTGCGGTCGCGGCCGCTCACTTGTACAGCTCATCCA |
