Fig. 1 | Scientific Reports

Fig. 1

From: Quantitative analysis of myeloid cell patterns and immunosuppressive enzyme (IDO, ARG1) expression in colorectal cancer pulmonary metastases and corresponding primary tumours

Fig. 1

Multiplex immunohistochemistry assay and machine learning-based image analysis. (A) Examples of each marker included in the assay separately and merged into pseudo-coloured multiplexed immunohistochemistry image. Haematoxylin (blue background staining) was used to align the cell nuclei. (B) Machine learning based image analysis for detecting and classifying tissue compartments and cells in QuPath. Tissue compartments were classified into tumour epithelium and stromal areas. Cells were classified into monocytic cells, granulocytes, mast cells, tumour cells, and other cells.

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