Fig. 4
From: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics
NMR analysis of the interaction between the RBD and tobramycin. (a) 1H-15N HSQC spectra of uniformly 15N-labeled RBD (100 μM) in the absence (black) and presence (red) of tenfold molar excess of tobramycin are overlaid. Signals that disappeared upon addition of tobramycin are indicated by boxes and labeled in blue. Asterisks indicate signals without assignments. (b) Residues with disappeared or significantly reduced signals upon addition of tenfold molar excess of tobramycin (as shown in a) were mapped on the structure of the RBD in complex with the ACE2 peptidase domain (PDB ID: 6m0j). RBD and ACE2 are shown as cartoon and surface representations, respectively. Residues not analyzed are colored black.
