Abstract
High physical activity often results in complex and ambiguous proteomic changes. This study aimed to analyze possible changes in the composition and content of proteins in plasma samples of elite athletes from sports with various activity levels and intensity. We performed proteomic profiling of blood plasma from 93 elite athletes with over eight years of continuous professional experience. High-resolution tandem mass spectra obtained in the data-independent acquisition mode were analyzed using the PLGS algorithm against a library of known protein sequences, and the PowerNovo algorithm for de novo protein sequencing. Combining the two protein identification strategies improved the completeness of the analysis and expanded the number of identified proteins. We identified changes in levels of circulating proteins that distinguish the high-intensity group from other groups by proteins involved in immune response, lipid transport and metabolism, and oxygen and iron transport. Changes in protein levels in biological samples of professional athletes may be associated with the training load intensity and type of sport.
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Data availability
The datasets generated and analysed during the current study are available in the MassIVE repository, full member of the ProteomeXchange consortium – https://massive.ucsd.edu/ProteoSAFe/QueryPXD?id=PXD067642.
Abbreviations
- MaxO2:
-
Maximal oxygen uptake
- PLGS:
-
Protein Lynx Global Server
- MS:
-
Mass spectrometry
- CROSSL:
-
C-Terminal telopeptides of type I collagen
- A2M:
-
Alpha-2-macroglobulin
- APOH:
-
Apolipoprotein H (Beta-2-glycoprotein I)
- ApoD:
-
Apolipoprotein D
- ALT:
-
Alanine aminotransferase
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The work was performed within the framework of the Program for Basic Research in the Russian Federation for a long-term period (2021–2030) (125020701771-5).
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Conceptualization Denis V. Petrovskiy, Arthur T. Kopylov and Anna L. Kaysheva; methodology, Kristina A. Malsagova, Tatiana V. Butkova, and Ksenia A. Yurku; software, Kirill S. Nikolsky and Denis V. Petrovskiy; validation Kirill S. Nikolsky, Denis V. Petrovskiy and Arthur T. Kopylov; formal analysis, Valeriya I. Nakhod and Vladimir R. Rudnev; data curation, Alexander A. Izotov, Anastasiia S. Bukreeva. and Evgenii I. Balakin; writing – original draft preparation, Liudmila I. Kulikova and Kirill S. Nikolsky; writing—review and editing, Arthur T. Kopylov and Vasiliy I. Pustovoyt. All authors have read and agreed to the published version of the manuscript.
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The study was approved by the Board for Ethical Questions in A. I. Burnazyan State Research Center of the FMBA of Russia (Protocol No. 40 from 18.11.2020) according to the principles expressed in the Declaration of Helsinki.
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Malsagova, K.A., Butkova, T.V., Nikolsky, K.S. et al. Changes in the proteomic profile of athletes’ plasma associated with exercise intensity. Sci Rep (2026). https://doi.org/10.1038/s41598-026-44729-5
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DOI: https://doi.org/10.1038/s41598-026-44729-5


