Fig. 3
Inhibition of Numb expression enhanced radiation-induced senescence in intestinal epithelial cells. (A-C) Immunohistochemical staining of intestinal sections and western blotting using intestinal mucosal extracts to assess active caspase-3, γH2AX, and p21 expression on days 3 and 7 after 13 Gy WAI. Representative images are displayed. Scale bar = 100 μm. Quantitative analysis of the western blotting results are presented in the form of statistical bars on the right panel of the corresponding immunohistochemistry images. n = 3 mice in each group. (D) Frozen sections of the intestine were stained for senescence-associated β-galactosidase (SA-β-gal) activity in intestinal epithelial cells on days 3 and 7 following 13 Gy WAI. SA- β-galactosidase levels were quantified via western blotting using intestinal mucosal extracts and are presented on the right panel. n = 3 mice in each group. The values are presented as the means ± SEMs. **P < 0.01, *P < 0.05.
