Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Advertisement

Scientific Reports
  • View all journals
  • Search
  • My Account Login
  • Content Explore content
  • About the journal
  • Publish with us
  • Sign up for alerts
  • RSS feed
  1. nature
  2. scientific reports
  3. articles
  4. article
mRNA profiling of mesenchymal stem cell-derived exosomes reveals their function in accelerating wound healing
Download PDF
Download PDF
  • Article
  • Open access
  • Published: 21 March 2026

mRNA profiling of mesenchymal stem cell-derived exosomes reveals their function in accelerating wound healing

  • Uyen Thi Trang Than1,2,
  • Hoai Thi Thanh Nguyen3,
  • Quang Minh Dang1,
  • Thu-Huyen Nguyen1,
  • Nhung Thi My Hoang3,
  • Thanh Hong Nguyen1,2,
  • Duc Minh Vu1,2,
  • Huong Thi Phi3,
  • Quynh Manh Luu3,
  • Xuan-Hai Do4,
  • Huy-Hoang Dao2,
  • Xuan-Hung Nguyen1,2 &
  • …
  • Nam Hoang Nguyen3 

Scientific Reports , Article number:  (2026) Cite this article

  • 940 Accesses

  • Metrics details

We are providing an unedited version of this manuscript to give early access to its findings. Before final publication, the manuscript will undergo further editing. Please note there may be errors present which affect the content, and all legal disclaimers apply.

Subjects

  • Cell biology
  • Molecular biology
  • Stem cells

Abstract

Extracellular vesicles (EVs) are emerging as innovative tools for regenerative and therapeutic applications, including wound healing, owing to their ability to encapsulate bioactive agents from their parent cells. In this study, we profiled the transcriptome of umbilical cord mesenchymal stem cell (UCMSC)-derived exosomes (EXs) using RNA-seq and explored the functional roles of their transcriptome, particularly in cutaneous wound repair. We detected 4,578 protein-coding genes in UCMSC-derived EXs, of which 2,004 were upregulated, and 2,574 were downregulated relative to their secreting cells. Notably, many EX-enriched genes were associated with wound-healing biology, and pathway analysis revealed that upregulated exosomal genes were involved in GO terms and KEGG pathways related to DNA replication, ribosome function, cell cycle regulation, and pyrimidine metabolism. To validate UCMSC-EX’s capability for wound healing predicted through in silico analyses, we further assessed EX penetration into the dermis, cellular uptake, and therapeutic efficacy in a burned mouse model. UCMSC-derived EXs efficiently penetrated human dermal tissue, were internalized by fibroblasts, and promoted fibroblast and keratinocyte proliferation and migration in 2D culture. In vivo, EX treatment accelerated wound closure, particularly during the early stages of healing. Overall, our findings demonstrate selective mRNA enrichment in UCMSC-derived EXs and highlight their promising therapeutic potential in cutaneous wound healing.

Data availability

All data generated in this study are provided in the articles and available in online supplementary files. All RNA sequencing data reported in this publication have been deposited in NCBI’s Gene Expression Omnibus and are accessible through the GEO accession number: GSE252017.

Abbreviations

EVs:

Extracellular vesicles

EXs:

Exosomes

MSCs:

Mesenchymal stem cells

UCMSCs:

Umbilical cord-derived mesenchymal stem cells

GO:

Gene ontology

KEGG:

Kyoto encyclopedia of genes and genomes

DEGs:

Differentially expressed genes

GAGE:

Generally applicable gene-set enrichment

DMEM/F12:

Dulbecco’s modified eagle medium/Ham’s F-12

FBS:

Fetal bovine serum

MVs:

Microvesicles

PBS:

Phosphate buffer saline

BCA:

Bicinchoninic acid

PVDF:

Polyvinylidene fluoride

TBST:

Tris-buffered saline with tween® 20 

NTA:

Nanoparticle tracking analysis

TEM:

Transmission electron microscopy

FDR:

False discovery rate

BH:

Benjamini and Hochbergw

References

  1. Liangsupree, T., Multia, E. & Riekkola, M.-L. Modern isolation and separation techniques for extracellular vesicles. J. Chromatogr. A 1636, 461773 (2021).

    Google Scholar 

  2. Zhang, H. et al. Exosome-induced regulation in inflammatory bowel disease. Frontiers Immunol. 10, 1464 (2019).

    Google Scholar 

  3. Boriachek, K. et al. Biological functions and current advances in isolation and detection strategies for exosome nanovesicles. Small 14, 1702153 (2018).

    Google Scholar 

  4. Cañas, J. A., Sastre, B., Rodrigo-Muñoz, J. M. & del Pozo, V. Exosomes: A new approach to asthma pathology. Clin. Chim. Acta 495, 139–147 (2019).

    Google Scholar 

  5. Valadi, H. et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 9, 654–659 (2007).

    Google Scholar 

  6. Rahmati, S., Shojaei, F., Shojaeian, A., Rezakhani, L. & Dehkordi, M. B. An overview of current knowledge in biological functions and potential theragnostic applications of exosomes. Chem. Phys. Lipids 226, 104836 (2020).

    Google Scholar 

  7. Dai, J. et al. Exosomes: Key players in cancer and potential therapeutic strategy. Signal Transduct. Target. Ther. 5145. (2020).

    Google Scholar 

  8. Phinney, D. G. & Pittenger, M. F. Concise review: MSC-derived exosomes for cell-free therapy. Stem Cells 35, 851–858 (2017).

    Google Scholar 

  9. Lee, B.-C., Kang, I. & Yu, K.-R. Therapeutic features and updated clinical trials of mesenchymal stem cell (MSC)-derived exosomes. J. Clin. Med. 10, 711 (2021).

    Google Scholar 

  10. Hoang, D. H. et al. Differential wound healing capacity of mesenchymal stem cell-derived exosomes originated from bone marrow, adipose tissue and umbilical cord under serum- and xeno-free condition. Front. Mol. Biosci. 7, 119 (2020).

    Google Scholar 

  11. Mendt, M., Rezvani, K. & Shpall, E. Mesenchymal stem cell-derived exosomes for clinical use. Bone Marrow Transplant. 54, 789–792 (2019).

    Google Scholar 

  12. Wang, Z.-g et al. Comprehensive proteomic analysis of exosomes derived from human bone marrow, adipose tissue, and umbilical cord mesenchymal stem cells. Stem Cell Res. Ther. 11, 511 (2020).

    Google Scholar 

  13. Joorabloo, A. & Liu, T. Engineering exosome-based biomimetic nanovehicles for wound healing. J. Control. Release 356, 463–480 (2023).

    Google Scholar 

  14. Han, X. et al. Exosomes derived from autologous dermal fibroblasts promote diabetic cutaneous wound healing through the Akt/β-catenin pathway. Cell Cycle 20, 616–629 (2021).

    Google Scholar 

  15. Dalirfardouei, R., Jamialahmadi, K., Jafarian, A. H. & Mahdipour, E. Promising effects of exosomes isolated from menstrual blood-derived mesenchymal stem cell on wound-healing process in diabetic mouse model. J. Tissue Eng. Regen. Med. 13, 555–568 (2019).

    Google Scholar 

  16. Belvedere, R. et al. Mesoglycan induces the secretion of microvesicles by keratinocytes able to activate human fibroblasts and endothelial cells: A novel mechanism in skin wound healing. Eur. J. Pharmacol. 869, 172894 (2020).

    Google Scholar 

  17. Iqbal, Z. et al. Exosome for mRNA delivery: Strategies and therapeutic applications. J. Nanobiotechnol. 22, 395 (2024).

    Google Scholar 

  18. Ma, H., Zhang, S., Xu, Y., Zhang, R. & Zhang, X. Analysis of differentially expressed microRNA of TNF-α-stimulated mesenchymal stem cells and exosomes from their culture supernatant. Arch. Med. Sci. 14, 1102–1111 (2018).

    Google Scholar 

  19. Yang, B.-c et al. Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis. Biochem. Biophys. Res. Commun. 524, 883–889 (2020).

    Google Scholar 

  20. Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014).

    Google Scholar 

  21. O’Grady, T. et al. Sorting and packaging of RNA into extracellular vesicles shape intracellular transcript levels. BMC Biol. 20, 72 (2022).

    Google Scholar 

  22. Bader, G. D. & Hogue, C. W. V. An automated method for finding molecular complexes in large protein interaction networks. BMC Bioinformatics 4, 2 (2003).

    Google Scholar 

  23. Li, H. T. et al. Diagnostic and prognostic value of MCM3 and its interacting proteins in hepatocellular carcinoma. Oncol. Lett. 20, 308 (2020).

    Google Scholar 

  24. Valverde, L. et al. MCM3: A novel proliferation marker in oral squamous cell carcinoma. Appl. Immunohistochem. Mol. Morphol. 26, 120–125 (2018).

    Google Scholar 

  25. Gao, Z. et al. PLK1 promotes proliferation and suppresses apoptosis of renal cell carcinoma cells by phosphorylating MCM3. Cancer Gene Ther. 27, 412–423 (2020).

    Google Scholar 

  26. Human Gene ATP5F1C (ENST00000356708.12) from GENCODE V47.

  27. Zhang, B. et al. Exosomes from human umbilical cord mesenchymal stem cells: Identification, purification, and biological characteristics. Stem Cells Int. 2016, 1929536 (2016).

    Google Scholar 

  28. Zhang, B. et al. HucMSC-exosome mediated-Wnt4 signaling is required for cutaneous wound healing. Stem Cells 33, 2158–68 (2015).

    Google Scholar 

  29. Kim, Y. J. et al. Exosomes derived from human umbilical cord blood mesenchymal stem cells stimulates rejuvenation of human skin. Biochem. Biophys. Res. Commun. 493, 1102–1108 (2017).

    Google Scholar 

  30. Nikfarjam, S., Rezaie, J., Zolbanin, N. M. & Jafari, R. Mesenchymal stem cell derived-exosomes: A modern approach in translational medicine. J. Transl. Med. 18449. (2020).

    Google Scholar 

  31. Shabbir, A., Coz, A., Rodriguez, L., Salgado, M. & Badiavas, E. Mesenchymal stem cell exosomes induce the proliferation and migration of normal and chronic wound fibroblasts, and enhance angiogenesis in vitro. Stem Cells Development 24(14), 1635–1647 (2015).

    Google Scholar 

  32. Than, U. T. T., Guanzon, D., Leavesley, D. & Parker, T. Association of extracellular membrane vesicles with cutaneous wound healing. Int. J. Mol. Sci. https://doi.org/10.3390/ijms18050956 (2017).

    Google Scholar 

  33. Zhu, B. et al. Stem cell-derived exosomes prevent aging-induced cardiac dysfunction through a novel exosome/lncRNA MALAT1/NF-kappaB/TNF-alpha signaling pathway. Oxid Med Cell Longev 9739258 (2019). (2019).

  34. Hong, P., Yang, H., Wu, Y., Li, K. & Tang, Z. The functions and clinical application potential of exosomes derived from adipose mesenchymal stem cells: A comprehensive review. Stem Cell. Res. Ther. 10, 242 (2019).

    Google Scholar 

  35. Than, U. T. T., Guanzon, D., Broadbent, J. A., Parker, T. J. & Leavesley, D. I. Deep sequencing microRNAs from extracellular membrane vesicles revealed the association of the vesicle cargo with cellular origin. Int. J. Mol. Sci. 21, 1141 (2020).

    Google Scholar 

  36. Sonnemann, K. J. & Bement, W. M. Wound repair: Toward understanding and integration of single-cell and multicellular wound responses. Annu. Rev. Cell Dev. Biol. 27, 237–63 (2011).

    Google Scholar 

  37. Chemnitz, J. et al. The acidic protein rich in leucines Anp32b is an immunomodulator of inflammation in mice. Sci. Rep. 9, 4853 (2019).

    Google Scholar 

  38. Theisen, U., Straube, E. & Straube, A. Directional persistence of migrating cells requires Kif1C-mediated stabilization of trailing adhesions. Dev. Cell. 23, 1153–1166 (2012).

    Google Scholar 

  39. Kopp, P. et al. The kinesin KIF1C and microtubule plus ends regulate podosome dynamics in macrophages. Mol. Biol. Cell 17, 2811–23 (2006).

    Google Scholar 

  40. Murray, D., Horgan, G., Macmathuna, P. & Doran, P. NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer. Br. J. Cancer. 99, 1322–1329 (2008).

    Google Scholar 

  41. Williamson, R. C. et al. Coronin-1C and RCC2 guide mesenchymal migration by trafficking Rac1 and controlling GEF exposure. J. Cell Sci. 127, 4292–307 (2014).

    Google Scholar 

  42. Olaso, E. et al. Discoidin domain receptor 2 regulates fibroblast proliferation and migration through the extracellular matrix in association with transcriptional activation of matrix metalloproteinase-2. J. Biol. Chem. 277, 3606–3613 (2002).

    Google Scholar 

  43. Olaso, E., Lin, H. C., Wang, L. H. & Friedman, S. L. Impaired dermal wound healing in Discoidin domain receptor 2-deficient mice associated with defective extracellular matrix remodeling. Fibrogenesis Tissue Repair. 4, 5 (2011).

    Google Scholar 

  44. Lan, H. et al. Pro-angiogenic role of ZEB1 in skin wound healing by upregulating VEGFA via microRNA-206 suppression. Exp. Dermatol. 31, 1392–1401 (2022).

    Google Scholar 

  45. Zhang, X., Wang, M., Zhang, Y., Yang, J. & Duan, W. Knockdown of CENPU inhibits cervical cancer cell migration and stemness through the FOXM1/Wnt/β-catenin pathway. Tissue Cell. 81, 102009 (2023).

    Google Scholar 

  46. Bian, D., Wu, Y., Song, G., Azizi, R. & Zamani, A. The application of mesenchymal stromal cells (MSCs) and their derivative exosome in skin wound healing: A comprehensive review. Stem Cell Res. Ther. 13, 24 (2022).

    Google Scholar 

  47. Yang, Y. et al. Umbilical cord mesenchymal stem cell-derived exosomes promote wound healing and skin regeneration via the regulation of inflammation and angiogenesis. Front. Bioeng. Biotechnol. 13, 1641709 (2025).

    Google Scholar 

  48. Zhu, Z. et al. Exosomes derived from umbilical cord mesenchymal stem cells treat cutaneous nerve damage and promote wound healing. Front. Cell. Neurosci. 16, 913009 (2022).

    Google Scholar 

  49. Lo Sicco, C. et al. Mesenchymal stem cell-derived extracellular vesicles as mediators of anti-inflammatory effects: Endorsement of macrophage polarization. Stem Cells Transl. Med. 6, 1018–1028 (2017).

    Google Scholar 

  50. Xun, C., Deng, H., Zhao, J., Ge, L. & Hu, Z. Mesenchymal stromal cell extracellular vesicles for multiple sclerosis in preclinical rodent models: A meta-analysis. Front. Immunol. https://doi.org/10.3389/fimmu.2022.972247 (2022).

    Google Scholar 

  51. Chinwalla, A. T. et al. Initial sequencing and comparative analysis of the mouse genome. Nature 420, 520–562 (2002).

    Google Scholar 

  52. Howe, K. L. et al. Ensembl 2021. Nucleic Acids Res. 49, D884–D891 (2020).

    Google Scholar 

  53. Li, Q. et al. Extracellular vesicle-based mRNA therapeutics and vaccines. Exploration 5, 20240109 (2025).

    Google Scholar 

  54. Li, J., Chen, S., Wang, H., Wang, W. & Liu, Y. Mesenchymal stem cells derived EXO-miR3671 delivery promoted angiogenesis and accelerated wound healing of diabetes. Stem Cells https://doi.org/10.1093/stmcls/sxag008 (2026).

    Google Scholar 

  55. El Andaloussi, S., Mäger, I., Breakefield, X. O. & Wood, M. J. A. Extracellular vesicles: Biology and emerging therapeutic opportunities. Nat. Rev. Drug Discov. 12, 347–357 (2013).

    Google Scholar 

  56. Le, H. M. et al. Differential development of umbilical cord-derived mesenchymal stem cells during long-term maintenance in fetal bovine serum-supplemented medium and xeno- and serum-free culture medium. Cell. Reprogram. 23, 359–369 (2021).

    Google Scholar 

  57. Vu, D. M. et al. Effects of extracellular vesicles secreted by TGFβ-stimulated umbilical cord mesenchymal stem cells on skin fibroblasts by promoting fibroblast migration and ECM protein production. Biomedicines 10, 1810 (2022).

    Google Scholar 

  58. Dao, H. H. et al. Manufacturing exosomes for wound healing: Comparative analysis of culture media. PLoS One. 19, e0313697 (2024).

    Google Scholar 

  59. Nguyen, M. Q., Nguyen, D. D. & Than, U. T. T. The capacity of Wharton’s jelly to prolong the survival of skin tissues ex vivo. VNU J. Science: Nat. Sci. Technology https://doi.org/10.25073/2588-1140/vnunst.5593 (2024).

    Google Scholar 

  60. Andrews, S. & FastQC A quality control tool for high throughput sequence data Online at: (2010). https://www.bioinformatics.babraham.ac.uk/projects/fastqc/

  61. Martin, M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J. 17, 3 (2011).

    Google Scholar 

  62. Kim, D., Paggi, J. M., Park, C., Bennett, C. & Salzberg, S. L. Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype. Nat. Biotechnol. 37, 907–915 (2019).

    Google Scholar 

  63. Zerbino, D. R. et al. Ensembl 2018. Nucleic Acids Res. 46, D754–D761 (2017).

    Google Scholar 

  64. Li, H. et al. The sequence alignment/Map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).

    Google Scholar 

  65. Liao, Y., Smyth, G. K. & Shi, W. FeatureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics 30, 923–930 (2013).

    Google Scholar 

  66. Ewels, P., Magnusson, M., Lundin, S. & Käller, M. MultiQC: Summarize analysis results for multiple tools and samples in a single report. Bioinformatics 32, 3047–3048 (2016).

    Google Scholar 

  67. Hita, A. et al. MGcount: a total RNA-seq quantification tool to address multi-mapping and multi-overlapping alignments ambiguity in non-coding transcripts. BMC Bioinform. 23, 1–21 (2022).

    Google Scholar 

  68. Blighe, K., Rana, S. & Lewis, M. EnhancedVolcano: publication-ready volcano plots with enhanced colouring and labeling. (2021). https://github.com/kevinblighe/EnhancedVolcano

  69. Warnes, G. R. et al. Gplots: various R programming tools for plotting Data. (2025). https://cran.r-project.org/web/packages/gplots/index.html

  70. Yu, G., Wang, L. G., Han, Y. & He, Q. Y. Cluster profiler: An R package for comparing biological themes among gene clusters. OMICS A J. Integr. Biol. 16, 284–7 (2012).

    Google Scholar 

  71. Luo, W., Friedman, M. S., Shedden, K., Hankenson, K. D. & Woolf, P. J. GAGE: Generally applicable gene set enrichment for pathway analysis. BMC Bioinformatics 10, 1–17 (2009).

    Google Scholar 

  72. Kanehisa, M. & Sato, Y. KEGG mapper for inferring cellular functions from protein sequences. Protein Sci. 29, 28–35 (2020).

    Google Scholar 

Download references

Acknowledgements

We sincerely thank Dr. Nguyen Thi Nhan for their valuable bioinformatics analysis and review advice.

Funding

This project was funded by the VinIF project code VINIF.2021.DA00193.

Author information

Authors and Affiliations

  1. Vinmec-VinUni Institute of Immunology College of Health Sciences , VinUniversity, 100000, Hanoi, Vietnam

    Uyen Thi Trang Than, Quang Minh Dang, Thu-Huyen Nguyen, Thanh Hong Nguyen, Duc Minh Vu & Xuan-Hung Nguyen

  2. Vinmec Hi-Tech Center Vinmec Healthcare System , 100000, Hanoi, Vietnam

    Uyen Thi Trang Than, Thanh Hong Nguyen, Duc Minh Vu, Huy-Hoang Dao & Xuan-Hung Nguyen

  3. VNU University of Science Vietnam National University , 100000, Hanoi , Vietnam

    Hoai Thi Thanh Nguyen, Nhung Thi My Hoang, Huong Thi Phi, Quynh Manh Luu & Nam Hoang Nguyen

  4. Department of Practical and Experimental Surgery , Military Medical University , 160 Phung Hung Street, Phuc La, Ha Dong, Hanoi, Vietnam

    Xuan-Hai Do

Authors
  1. Uyen Thi Trang Than
    View author publications

    Search author on:PubMed Google Scholar

  2. Hoai Thi Thanh Nguyen
    View author publications

    Search author on:PubMed Google Scholar

  3. Quang Minh Dang
    View author publications

    Search author on:PubMed Google Scholar

  4. Thu-Huyen Nguyen
    View author publications

    Search author on:PubMed Google Scholar

  5. Nhung Thi My Hoang
    View author publications

    Search author on:PubMed Google Scholar

  6. Thanh Hong Nguyen
    View author publications

    Search author on:PubMed Google Scholar

  7. Duc Minh Vu
    View author publications

    Search author on:PubMed Google Scholar

  8. Huong Thi Phi
    View author publications

    Search author on:PubMed Google Scholar

  9. Quynh Manh Luu
    View author publications

    Search author on:PubMed Google Scholar

  10. Xuan-Hai Do
    View author publications

    Search author on:PubMed Google Scholar

  11. Huy-Hoang Dao
    View author publications

    Search author on:PubMed Google Scholar

  12. Xuan-Hung Nguyen
    View author publications

    Search author on:PubMed Google Scholar

  13. Nam Hoang Nguyen
    View author publications

    Search author on:PubMed Google Scholar

Contributions

The conception and design of the study: UTTT, NHN, NTMH, and X-HN; Data collection: UTTT, HTTN, QMD, DMV, T-HN, NTMH, THN, X-HN, X-HD, HHD, HTP, QML; Analysis and interpretation of data: UTTT, HTTN, QMD, DMV, THN, NTMH, THN, X-HN, X-HD, HHD, HTP, QML; Manuscript drafting: UTTT, THN, QMD; Manuscript revising, UTTT, X-HN, NTMH, NHN; Final approval: NHN; Funding acquisition: NHN and UTTT.

Corresponding author

Correspondence to Nam Hoang Nguyen.

Ethics declarations

Competing interests

The authors declare no competing interests.

Ethics approval and consent to participate

Ethical approval for the use of human MSCs from the umbilical cord and dermal fibroblasts was issued by the Vinmec International General Hospital Joint Stock Company’s ethics committee (Ethical approval number: 02/2022/CN-HĐĐĐ VMEC). The umbilical cord tissues were collected from three healthy donors aged 20 to 40, and skin tissues for fibroblasts were collected from women who had undergone plastic surgery. All donors signed written informed consent before donating their samples, and experimental protocols were performed in accordance with the relevant guidelines and regulations and approved by the ethics committee. For the use of animals and all experimental protocols involving animals, the study was approved by the Institutional Review Board at Dinh Tien Hoang Institute of Medicine. The Ethical approval number IRB-A 2203. We confirmed that all experiments and methods used in this study were performed in accordance with relevant regulations and the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guideline.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary Material 1 (download PDF )

Supplementary Material 2 (download DOCX )

Supplementary Material 3 (download DOCX )

Supplementary Material 4 (download DOCX )

Supplementary Material 5 (download DOCX )

Supplementary Material 6 (download DOCX )

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Than, U.T.T., Nguyen, H.T.T., Dang, Q.M. et al. mRNA profiling of mesenchymal stem cell-derived exosomes reveals their function in accelerating wound healing. Sci Rep (2026). https://doi.org/10.1038/s41598-026-45267-w

Download citation

  • Received: 17 November 2025

  • Accepted: 17 March 2026

  • Published: 21 March 2026

  • DOI: https://doi.org/10.1038/s41598-026-45267-w

Share this article

Anyone you share the following link with will be able to read this content:

Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative

Keywords

  • Umbilical mesenchymal stem cell-derived exosomes
  • RNA-seq
  • Exosome penetration
  • Cutaneous wound healing
Download PDF

Advertisement

Explore content

  • Research articles
  • News & Comment
  • Collections
  • Subjects
  • Follow us on Facebook
  • Follow us on X
  • Sign up for alerts
  • RSS feed

About the journal

  • About Scientific Reports
  • Contact
  • Journal policies
  • Guide to referees
  • Calls for Papers
  • Editor's Choice
  • Journal highlights
  • Open Access Fees and Funding

Publish with us

  • For authors
  • Language editing services
  • Open access funding
  • Submit manuscript

Search

Advanced search

Quick links

  • Explore articles by subject
  • Find a job
  • Guide to authors
  • Editorial policies

Scientific Reports (Sci Rep)

ISSN 2045-2322 (online)

nature.com footer links

About Nature Portfolio

  • About us
  • Press releases
  • Press office
  • Contact us

Discover content

  • Journals A-Z
  • Articles by subject
  • protocols.io
  • Nature Index

Publishing policies

  • Nature portfolio policies
  • Open access

Author & Researcher services

  • Reprints & permissions
  • Research data
  • Language editing
  • Scientific editing
  • Nature Masterclasses
  • Research Solutions

Libraries & institutions

  • Librarian service & tools
  • Librarian portal
  • Open research
  • Recommend to library

Advertising & partnerships

  • Advertising
  • Partnerships & Services
  • Media kits
  • Branded content

Professional development

  • Nature Awards
  • Nature Careers
  • Nature Conferences

Regional websites

  • Nature Africa
  • Nature China
  • Nature India
  • Nature Japan
  • Nature Middle East
  • Privacy Policy
  • Use of cookies
  • Legal notice
  • Accessibility statement
  • Terms & Conditions
  • Your US state privacy rights
Springer Nature

© 2026 Springer Nature Limited

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing