Fig. 3
The target of HZKL in inhibiting cellular pyroptosis in UC is screened. (A) Western blot analysis of ZBP1, CD55, S100A8, and LCN2 protein expression in colonic tissues, with GAPDH as a loading control. (B–E) Densitometric quantification of protein expression levels shown in (A) (mean ± SEM, n = 3 independent biological replicates). (F–I) Quantitative real-time PCR analysis of ZBP1, CD55, S100A8, and LCN2 mRNA expression levels in colonic tissues (mean ± SEM, n = 3 independent biological replicates). (J,K) Immunofluorescence co-localization analysis showing LCN2 (green) with ASC or NLRP3 (red) in colonic tissues; nuclei were counterstained with DAPI (blue). Yellow signals indicate co-localization (scale bar: 50 μm). Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
