Table 1 Some important but unresolved questions on amphibian welfare
1 | Is MS222 an appropriate chemical for euthanasia of amphibians? |
2 | What biomarkers of welfare are applicable to amphibians? |
3 | What is the efficacy of current analgesia and anaesthesia? |
4 | Can Xenopus superovulation / general breeding protocols be refined? |
5 | Are corticosterone readouts valid measures of welfare? |
6 | Can established preference tests be created for amphibians? |
7 | What non-invasive monitoring methodologies exist for these animals? |
8 | Have species-specific baseline behaviours been documented? |
9 | What is the ideal nutritional composition of a regulated amphibian diet? |
10 | Is live food a necessity in an amphibian diet? |
11 | How does water quality and composition affect amphibian welfare? |
12 | What (if any) are the health benefits of ultraviolet light for amphibians? |
13 | Does providing a gradient in the physical captive environment of amphibians (to enable choice of microenvironment) improve health and welfare? |