Research articles

This study introduces IntelliProfiler, a software workflow for radio frequency identification-based analysis of locomotor activity and social spacing in mice, revealing sex, age and autism spectrum disorder model effects, enabling scalable, long-term behavioral phenotyping.

The authors analyzed four melioidosis studies in mice to identify refined humane endpoints (25% weight loss and/or clinical score ≥5) that improve animal welfare while maintaining study integrity.

This study explores how biological, technical and experimental factors affect gut microbiota variability in mouse fecal samples. Repeated sampling improves statistical power but raises costs, highlighting the practical limitations of this approach.

This study presents a bronchoscopically induced lung cancer model in Oncopigs with inducible KRAS and TP53 mutations. Showing high induction rates and human-like tumoral gene expression, the model offers a translational complement to rodent systems.

This study shows an efficient approach for in vitro production of marmoset embryos following an optimized ovarian stimulation protocol combined with laparoscopic ovum pick-up, potentially facilitating reproductive biotechnology in marmosets.

This Article presents a new digital biomarker of diabetes, the Urination Index, to monitor bedding moisture in mice. This noninvasive method reduces the need for invasive blood glucose tests, improving animal welfare and data reliability.

This study shows that restricting irradiation to one hemisphere of rat brains causes less cognitive impairment and brain damage, while improving clinical relevance, compared with the whole-brain irradiation model commonly used in radiotherapy research.

This study assesses various methods and timings for perfusion of fixatives to enhance brain histology, addressing ethical dilemmas associated with ante-mortem transcardiac perfusion and the lack of data on tissue quality using different techniques.

The authors developed a new behavior classifier using color-based body segmentation that can capture body parts, track body movements and detect subtle behavioral phenotypes in freely moving mice.

The study compares acclimatization protocols for metabolic cages and found that the standard housing at 23 °C impacts mice’s physiology and welfare owing to cold stress. However, thermoneutral housing provides a simple solution to improve mouse welfare.

By assessing different clinical, behavioral and welfare parameters, the authors demonstrate that the oral administration of tramadol is more suitable than metamizole as on-demand analgesia in mouse models of acute lymphoblastic leukemia.

Jovanovic et al. show that a gel-based diet can be used as a minimally invasive alternative system for the delivery of small molecules for microglia depletion and inducible Cre recombination in mice.

The authors report the development of a new rat model of forelimb ischemia–reperfusion injury, characterized by motor coordination deficits, impairment of neuromuscular transmission and serum biochemical and inflammatory changes.

The study reveals that long-term anesthesia with ketamine–xylazine can induce hypoxia and mortality in mice, whereas oxygen supplementation mitigates these effects. As an alternative, isoflurane showed superior safety and fewer effects on the immune system.

The authors describe the second generation of the mutant methionyl tRNA synthetase mouse line, designed to increase mutant enzyme expression, enhance the labeling of nascent proteins and facilitate cell type-specific proteome identification.

The Article describes a minipig model of obesity-associated meta-inflammation, characterized by increased C-reactive protein levels and white blood cells, together with changes in the expression of genes related to inflammation and metabolism.

A new color vision optomotor response assay was evaluated for assessing red cone ablation in transgenic zebrafish. The results suggest that the assay can be useful for evaluating visual function and color vision in zebrafish with visual disorders.

This study uses a transgenic mouse line expressing Cre-inducible astro-hM3Dq to study astrocytes’ role in neuroinflammation and cognition without the use of viral transduction, mitigating the potential side effects associated with standard techniques.

This study analyzes the course of murine astrovirus infection and the immune response in multiple mouse strains to identify the best sentinel strain and the best diagnostic method when including murine astrovirus in health monitoring programs.

The authors compare the efficacy of injectable and oral broad-spectrum antibiotics on reducing bacteria from the upper respiratory tract of ferrets, before evaluating the effects of commensal bacteria reduction on influenza A virus infection.