Zhu, H. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-018-0318-7 (2018)
Need to get your gene editor to a specific tissue or organ in vivo? Magnets might do the trick, says a team of bioengineers from Rice University in Houston, Texas.
The researchers developed a novel delivery vehicle for a gene editor that consists of an inactivated baculoviral vector combined with magnetic nanoparticles. The vector is derived from a virus that infects the alfalfa looper moth and can efficiently carry large cargo, like lengthy CRISPR/Cas9 sequences, while the magnets can influence deep tissues without adverse effect. Once in the body (injected, in a mouse’s case, intravenously via the tail), you need only apply a small magnet near the desired editing site to release the editor there. The researchers demonstrate the system’s in vivo potential in mouse liver, spleen, and subcutaneous tumors.
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Neff, E.P. Magnetic delivery. Lab Anim 48, 47 (2019). https://doi.org/10.1038/s41684-018-0234-1
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DOI: https://doi.org/10.1038/s41684-018-0234-1