The application of genome-editing tools to generate point mutations in animal models is of particular value for precise disease modeling, but the PAM requirement of Cas enzymes is a critical limiting factor. Two new studies demonstrate that SpRY variant displays efficient genome editing in a nearly PAM-less manner in zebrafish, expanding the targeting scope of base editors in this model.
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Varshney, P., Varshney, G.K. Expanded precision genome-editing toolbox for human disease modeling in zebrafish. Lab Anim 51, 287–289 (2022). https://doi.org/10.1038/s41684-022-01076-y
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DOI: https://doi.org/10.1038/s41684-022-01076-y
