Fig. 9: Type I IFNs protect against WU23 intestinal disease in adult mice. | Lab Animal

Fig. 9: Type I IFNs protect against WU23 intestinal disease in adult mice.

From: Infection of neonatal mice with the murine norovirus strain WU23 is a robust model to study norovirus pathogenesis

Fig. 9

Groups of adult C57BL/6J (B6), Ifnar−/− and Ifnlr−/− mice (n = 12–25 per condition) were p.o. infected with 107 TCID50 units of MNV1, WU23, CR6 or mock inoculum and killed at 3 dpi. a, Stomachs were ligated, weighed, contents removed and then weighed again to calculate weight of stomach contents. b, To calculate small intestinal fluid accumulation, intestines were ligated into thirds. Each section was weighed and length measured to determine the weight-to-length ratios. Mice from three independent experiments were analyzed for all conditions. WT, wild type. c, Groups of adult mice of the indicated strains (n = 6 per condition) were p.o. infected with 107 TCID50 units of WU23. At 1 dpi, virus titers were determined in the indicated segments of the intestinal tract, spleen or liver by plaque assay. Data are presented as mean ± s.e.m.; P values were determined using two-sided ANOVA with corrections for multiple comparisons using Tukey’s test. Dashed line indicates limit of detection. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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