Fig. 2: Activities of vaccine-induced antibodies from Aotus following immunization.
a, The reactivity of vaccine-induced IgG 2 weeks after the last vaccination dose was assessed against PMV candidates PAMVAC (Pf-FCR3) (n = 9) and PRIMVAC (Pf-3D7) (n = 9) as well as recombinant antigens rID1-ID2a-M1010 (produced from the ID1-ID2a construct of VAR2CSAM1010 sequence) (n = 9) and rPfs25 (n = 13). b,c, Functional characterization of antibodies was evaluated for the ability of IgG to bind antigens expressed on the surface of the IEs by flow cytometry analysis (b), and for CSA-binding inhibition activity against different P. falciparum isolates (c). For each group of vaccinated monkeys, the geometric mean and 95% confidence interval of the antibody activity measured by ELISA and flow cytometry are shown (a and b). For BIA, the mean and s.e.m. are shown (c). Mann–Whitney test was used to compare activity of PMV-induced antibody to that of Pfs25 with P values indicated as *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.005 and ****P ≤ 0.001.
