Fig. 5: Dynamics of cross-inhibitory and cross-recognition activity of PMV-induced antibodies in Aotus monkeys in response to pregnancy malaria.
a–c, The levels of binding inhibition activity of the PMV-induced antibodies against CS2 and NF54 parasites in samples collected at D70 post-vaccination (a), pre-CS2 inoculation (b) and 4 weeks postpartum (c) are shown. The dashed lines represent 50% inhibition, and the gray-colored area in each graph defines labeled samples with inhibitory activity. d–f, The recognition levels of surface-expressed VAR2CSA on CS2 and NF54 IE by the PMV-induced antibodies was also analyzed at D70 post-vaccination (d), pre-CS2 inoculation (e) and 4 weeks postpartum (f), with dashed lines representing rMFI 1.2 and the gray-colored area highlighting flow-positive samples. In a and d, n = 9 for PAMVAC-immunized monkeys, n = 9 for PRIMVAC-immunized monkeys, n = 9 for ID1-ID2a_M1010-immunized monkeys and n = 13 for Pfs25-immunized monkeys. In b, c, e and f, n = 6 for PAMVAC-immunized monkeys, n = 5 for PRIMVAC-immunized monkeys, n = 4 for ID1-ID2a_M1010-immunized monkeys and n = 7 for Pfs25-immunized monkeys. Data for animals immunized with PAMVAC (Pf-FCR3) (blue circle), PRIMVAC (Pf-3D7) (open red square), rID1-ID2a-M1010 (green triangle) and rPfs25 (black diamond) are shown and samples with cross-recognition activity are labeled. Spearman (ρ) coefficients and P values are reported for each graph.
