Fig. 4: Video-tracking analysis of mice in MCs under different conditions.

a,b, A percentage of time spent in each zone (food zone, water zone, nest zone, on the grid) of the MC during the day (a) and night (b). c–e, The average number of activity counts (arbitrary unit) measured by the implanted HD-S11 transmitter during baseline (c), MC housing (d) and recovery (e). f, The total distance traveled during 12 h of MC housing (day: 7:00–19:00, night: 19:00–7:00). A two-way ANOVA with Šídák’s multiple comparison test was used to compare groups in c–f (the P values are indicated in the graph, n = 9–12). The videos were recorded and analyzed with Ethovision XT in c–f. For video analysis, only videos from the sampling day (day 4) were used. In c–f, the data are shown as ±s.d. For a and b, only the mean is shown. Single-housed mice were either continuously kept in MCs at 23 °C for 4 days (MC23, blue, n = 6♀ + 6♂), acclimatized for 3 h per day until day 4 (accMC23, orange, n = 5♀ + 6♂) or provided with a nest (nest-MC23, green, n = 6♀ + 6♂). Another group was housed in a thermoneutral environment in the MC at 30 °C (MC30, red, n = 6♀ + 6♂). The experiment was performed in ten batches according to the availability of animals and groups.