Fig. 5: Changes in body composition and ketone body concentration.
From: Thermoneutral environment improves mouse welfare and reduces stress in metabolic cages
a, Body composition was measured in nonimplanted mice before MC housing (HC23) and at the end of MC housing. b–d, Relative gene expression of Ucp1 (b), Fasn (c) and Ndufa (d) in BAT. BAT was collected immediately after echo MRI measurements. e, Ketone body concentration measured in a blood sample obtained by tail-vein puncture. The concentration was measured daily at 16:00 h while the mice were kept in a handling tube. A one-way ANOVA with uncorrected Fisher’s least significant difference test in a and two-way ANOVA with Tukey’s multiple comparisons in b–e statistical tests were performed (the P values are indicated in the graph) (n = 5–6). In a–e, the data are shown as ±s.d. Single-housed mice were either kept in MC at 23 °C for 4 days continuously (MC23, blue, n = 6♀ + 6♂) or in a thermoneutral environment in the MC at 30 °C (MC30, red, n = 6♀ + 6♂). Average of all differences in fat mass was calculated from the measured fat ratios before and after MC housing.
