Table 1 Summary of the fertility-relevant sperm functional characteristics and the methods commonly performed for their assessment
From: Beyond swimming: emerging parameters for predicting the fertility of mouse spermatozoa
Parameter | CASA | Brightfield microscopy | Fluorescence microscopy | Flow cytometry |
|---|---|---|---|---|
Concentration | ✓ | ✓ | ✓ | ✓ |
Morphology | ✓ | ✓ | ✓ | ✓ |
Motility | ✓ | ✓ | ||
Hyperactivated motility | ✓ | ✓ | ||
Plasma membrane integrity | ✓ | ✓ | ✓ | |
Acrosomal status | ✓ | ✓ | ✓ | |
Mitochondrial membrane potential | ✓ | ✓ | ||
Intracellular Ca2+ levels | ✓ | ✓ | ||
DNA integrity | ✓ | ✓ | ✓ |
