Fig. 5: STZ main study: high/low responder analysis.
a–e, Time course development (mean ± s.e.m.) in ‘STZ high’ responder (n = 4) and ‘STZ low’ responder (n = 4), and Ctrl cages (n = 6) of food intake (a), blood glucose (b), water intake (c), daily UI change (d) and UI (e) following STZ dosing (shaded area). The main effects and the interaction between groups and time were highly significant for all of a–e tested by repeated-measures two-way ANOVA adjusted with Tukey’s multiple comparisons test. The arrows denote the first significant divergence of ‘STZ high’ or ‘STZ low’ groups from Ctrl (P < 0.05). The black arrows indicate simultaneous divergence of ‘STZ high’ and ‘STZ low’ from Ctrl, while the divergence at different time points is indicated in arrows matching the group color. All other indicators of significance have been omitted for clarity. f, The average daily urination rate before (days 0–11) and after STZ (days 29–57) (mean ± s.e.m., group-wise ANOVA, P < 0.0001). g–i, The circadian change in UI (mean ± s.e.m.) before STZ (g), during STZ treatment (h) and after STZ (i). Significant differences from Ctrl for each STZ group are indicated with brackets in the group color, and purple bracket indicates differences between ‘STZ high’ and ‘STZ low’ (mean ± s.e.m., tested by repeated-measures two-way ANOVA adjusted with Tukey’s multiple comparisons test, P < 0.05). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
