Fig. 2: Nuclear wrinkles are not an artifact of tissue processing and allow nuclei to assume diverse shapes.
From: Extreme wrinkling of the nuclear lamina is a morphological marker of cancer
a Excess perimeter distribution for pooled ductal carcinoma in situ (DCIS) patient nuclei and pooled breast cancer nuclei. Mean values were calculated from DCIS (n = 1882) and invasive cancer (n = 4575) nuclei. Error bars present standard deviations. b Collage of nuclei observed in formalin-fixed paraffin-embedded (FFPE) DCIS tissue sample (left) and invasive cancer samples (right) stained for lamin B1. c Frozen normal and cancer breast tissue fixed with acetone and stained for lamin B1. Scale bar is 5 µm. d Top: FFPE tissue section exhibiting shrinkage in the form of empty spaces (visible in brightfield) stained for lamin B1 (yellow) and pan-cytokeratin (magenta). Scale bar is 20 µm for 60× and 5 µm for zoomed. Below are zoomed regions from the lamin B1 image above (gray). e FFPE tissue sections featuring adjacent smooth and wrinkled nuclei. Scale bar is 5 µm. All images were taken at 60×.
