Fig. 5: Mitochondrial function is dependent on Mg2+ entry in macrophage cells. | npj Precision Oncology

Fig. 5: Mitochondrial function is dependent on Mg2+ entry in macrophage cells.

From: Naltriben promotes tumor growth by activating the TRPM7-mediated development of the anti-inflammatory M2 phenotype

Fig. 5: Mitochondrial function is dependent on Mg2+ entry in macrophage cells.

A Spectrofluorimetric traces depicting permeabilized macrophages (Naïve, M1, M2) exposed to FCCP at the indicated time point to assess basal mMg2+. B Quantification of basal mMg2+ in permeabilized macrophages. C Spectrofluorimetric traces representing permeabilized macrophages (M1, M2) in the presence of 20 mM Mg2+ for 1 h exposed to FCCP at the indicated time point to assess basal mMg2+. D Quantification of basal mMg2+ in permeabilized macrophages in the presence of Mg2+. E, G, I Oxygen Consumption Rate (OCR) was analyzed in macrophages isolated from WT with and without Mg2+ for 1 hour for M0, M1, and M2 phenotypes. N = 3 performed in duplicate. F, H, J Basal, maximal respiration, and proton leak analysis of macrophages isolated from WT with and without Mg2+ for 1 hour. Mean ± SEM, n = 3, ***p < 0.001.

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