Fig. 1: A flow for the analysis process.

JPH Jiangsu Province Hospital, CHOL cholangiocarcinoma, GBC gallbladder cancer, NGS next-generation sequencing, PR partial response, SD_PD stable disease and progressive disease, PEA proximity extension assay, GO gene ontology, KEGG kyoto encyclopedia of gene and genomics, COG clusters of orthologous groups, PPI protein–protein interaction networks, ICB immune checkpoint blockade.