Fig. 7: TRIM65 regulates the glycolysis process through AMPK and HIF-1α pathway. | npj Precision Oncology

Fig. 7: TRIM65 regulates the glycolysis process through AMPK and HIF-1α pathway.

From: TRIM65 regulates glucose metabolic reprogramming to promote glioma cell proliferation via ubiquitination and degradation of AMPK

Fig. 7

A Western blot analysis of HIF-1α protein expression in U251 and U87 cells with TRIM65 knockdown or overexpression and then the related protein level was quantified. F Flag-tagged TRIM65, W wild-type TRIM65. B Representative IHC images of xenograft tumors with anti-TRIM65, anti-AMPK and anti-HIF-1α. Scale bar = 20 μm. Lactate production (C) and cell viability (D) were measured in U251 and U87 cells knocked down TRIM65 in the presence or absence of overexpressed HIF-1α. E Western blot analysis of HIF-1α protein expression in U251 and U87 cells treated as indicated. The related protein level was quantified. Data are presented as mean ± SD. The experiments were independently repeated at least three times and the data of one representative experiment was shown. *p < 0.05; **p < 0.01, ***p < 0.001.

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