Fig. 2: Single cell sequencing shows Gαi3 overexpression in epithelial cells of bladder cancer.

The UMAP (Uniform Manifold Approximation and Projection) plot of cell annotations derived from single-cell data of bladder cancer (GSE222315, A) illustrates the expression distribution of Gαi3. A dot plot showed the expression levels of cell type-specific marker genes across the identified cell clusters (B). The distribution is further visualized in UMAP plot (C), Dot plot (D) for normal (“NORM”) and bladder cancer (“BLCA”) samples. A violin plot highlights the expression levels of Gαi3 in normal (“NORM”) and bladder cancer (“BLCA”) epithelial cell subsets (E), with the Wilcoxon rank-sum test was performed. Volcano plot showing genes differentially expressed between Gαi3+ and Gαi3- epithelial cells (F). Red points are upregulated, blue are downregulated, grey are not significant (F). Top enriched GO Biological Process terms for the DEGs, sorted by adjusted P value (G). Top enriched KEGG pathways for the DEGs, sorted by adjusted P value (H).