Fig. 3: Experimental validation of YY1 regulation and miR-605-3p targeting in glioblastoma cells.
From: Deciphering the YY1/ lncRNA BLACAT1/miR-605-3p axis in glioblastoma: implications for therapy
A ChIP-qPCR experiments confirmed YY1 as an upstream transcription factor regulating the lncRNA BLACAT1 gene expression. B Dual-luciferase reporter assays validated the direct regulation of ITGA3, ARPC1B, FOSL1, H6PD, LMAN1, and PXN by miR-605-3p. C RT-qPCR results from various experimental groups demonstrated the downstream effects of YY1 knockout, miR-605-3p inhibitor transfection, and miR-605-3p mimic transfection. D Western blot analyses further supported the regulatory interactions, highlighting changes in protein expression levels. ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Each experiment was performed with three independent biological replicates.
