Fig. 2: Phenotypic and transcriptomic effects of CREB5 in BLBC cell models. | npj Precision Oncology

Fig. 2: Phenotypic and transcriptomic effects of CREB5 in BLBC cell models.

From: CREB5 promotes tumorigenicity and upregulates druggable cell surface modalities in basal-like breast cancer

Fig. 2

Proliferation assay showing median live cell count over 10 days for A MDA-MB-231 and B HCC1806 cell lines with either CREB5 or luciferase (LUC) overexpressed. C Tumorsphere formation assay results for MDA-MB-231 (top row) and HCC1806 (bottom row) cell lines with CREB5 (left panels) or luciferase overexpressed. Scale bar shown is 200 μm. Quantitative analysis of median D tumorsphere formation efficiency and E tumorsphere size (μm²). F Volcano plots illustrating changes in gene expression from RNA sequencing in MDA-MB-231 (left panels) and HCC1806 (right panels) cell lines, in 2D (top row) and 3D (bottom row) culture. Each dot represents a gene that either passes the significant thresholds for both the p-value and log-fold changes (red), for only the p-value (blue), or neither (gray). G Net enrichment scores (NES) from GSEA based on C6 oncogenic signatures depicted across cell lines in 3D culture. Red circles: significantly upregulated both cell lines. Orange circles: only significantly upregulated in the MDA-MB-231 cells. Purple circles: only significantly upregulated in the HCC1806 cells. Significance is based on FDR < 0.05.

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