Fig. 4: ZEB1 directly interacts with KAT2B and EP300 in a ZF-CP-dependent pattern. | npj Precision Oncology

Fig. 4: ZEB1 directly interacts with KAT2B and EP300 in a ZF-CP-dependent pattern.

From: ZEB1 and ALKBH5 coregulate CRABP2 and FABP5 and jointly determine the sensitivity of anaplastic thyroid cancers to gemcitabine and retinoic acid

Fig. 4: ZEB1 directly interacts with KAT2B and EP300 in a ZF-CP-dependent pattern.

A Co-IP assay with anti-Myc antibodies in ZEB1-FLAG- and WT-KAT2B-Myc- overexpressing THJ-16T cells followed by IB. B Co-IP assay with anti-Myc antibodies in ZEB1-FLAG- and WT-EP300-Myc- overexpressing THJ-16T cells followed by IB. C, D PLA and IF staining experiments were used to assess the binding requirements of the CP domain in ZEB1 to KAT2B and EP300. Scale bar, 50 µm. E Co-IP experiments demonstrated that the influence of EP300 overexpression on the interaction between ZEB1 and KAT2B. The data are shown as the means ± SD from three independent experiments. Images from the IB, PLA and IF staining experiments are representative of three independent experiments. *p < 0.05, **p < 0.01 indicate statistical significance.

Back to article page