Fig. 1: Deletion or mutation of the WW domain reverses the inhibitory effect of PCIF1 on glioma cell proliferation. | npj Precision Oncology

Fig. 1: Deletion or mutation of the WW domain reverses the inhibitory effect of PCIF1 on glioma cell proliferation.

From: The WW domain presents a promising target for the development of PCIF1 agonists in the treatment of glioma

Fig. 1: Deletion or mutation of the WW domain reverses the inhibitory effect of PCIF1 on glioma cell proliferation.

A Schematic representation of the structure of PCIF1-WT, PCIF1-ΔWW, and PCIF1-S54H constructs. B Western blot was used to confirm the overexpression efficiency of the indicated plasmids using a Flag antibody. C CCK-8 assay showed the effect of deletion or mutation of the WW domain on cell viability at different time points following transient transfection. D, E EdU assay showed the effect of deletion or mutation of the WW domain on cell proliferation following transient transfection. Quantitative analysis of the percentage of EdU-positive cells is shown in (D). Representative images of the EdU assay are shown in (E). **P < 0.01, ***P < 0.001, compared to the 3×Flag group; ##P < 0.01, ###P < 0.001, compared to the PCIF1-WT group. Scale bars: 200 μm.

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