Fig. 6: Overexpression of the WW domain promotes the degradation of endogenous PCIF1 protein and facilitates its translocation from the nucleus to the cytoplasm. | npj Precision Oncology

Fig. 6: Overexpression of the WW domain promotes the degradation of endogenous PCIF1 protein and facilitates its translocation from the nucleus to the cytoplasm.

From: The WW domain presents a promising target for the development of PCIF1 agonists in the treatment of glioma

Fig. 6: Overexpression of the WW domain promotes the degradation of endogenous PCIF1 protein and facilitates its translocation from the nucleus to the cytoplasm.

A Cells were treated with CHX (100 μg/mL) for the indicated time after transfecting the PCIF1(47–113) plasmid. Western blot was used to assess the degradation of the endogenous PCIF1 protein. B Cells were treated with MG132 (40 μM) and CQ (100 μg/mL) following overexpression of the PCIF1(47–113) plasmid, and the changes in endogenous PCIF1 protein levels were analyzed by Western blot. Band densities were quantified and shown as the ratio normalized to β-actin. C Immunofluorescence was performed to observe the localization of PCIF1 in U87 and T98G cells by confocal microscope imaging following transfecting the PCIF1(47–113) plasmid. Scale bars: 20 μm.

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