Fig. 5: An in vivo imaging-based candidate screen identifies GPR180/CG9304 as pro-glial infiltration in the QKI::RAF1 model.
From: Role of repulsive guidance signaling and GPR180 in pediatric low-grade glioma infiltration
a Schematic illustrating an in vivo screen of QKI::RAF1 flies to identify tumor-suppressing genes and oncogenes generated using Biorender.com. b In vivo images of the VNC and leg discs in third-instar larvae with mRFP in glia (red). The white outlines indicate the area of the leg discs, and the yellow arrows indicate the furthest point glia reach. If glia enter and migrate along the leg disc, this is the “migratory path.” Scale bar = 100 µm. c, d Quantification of glial migration in QKI::RAF1 flies with either an overexpression or knockdown. The only difference between (c) and (d) quantifications is that (c) includes all values, while d does not include any values of zero, which may indicate that the leg disc was not in position or obscured. For CD4-tdTomato, Nuf, Slit-D, Cap RNAi#1, Cap RNAi#2, Gol RNAi, Vrpl RNAi, Godzilla RNAi, Nrg RNAi, Prip RNAi, CG9304 RNAi#1, CG9304 RNAi#2, CG9304MI09328, N = 63, 26, 31, 32, 29, 32, 38, 19, 20, 68, 52, 66, 54 (c), and N = 37, 18, 21, 21, 20, 25, 12, 7, 12, 34, 8, 8,19 (d). All data are means ± SEM. The data were analyzed by one-way ANOVA followed by Dunn’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bar = 100 µm.
