Fig. 3: Immunohistology analysis of primary PBT samples and corresponding PDT cultures. | npj Precision Oncology

Fig. 3: Immunohistology analysis of primary PBT samples and corresponding PDT cultures.

From: Patient-derived tumoroids recapitulate the morphologic and molecular features of pediatric brain tumors

Fig. 3: Immunohistology analysis of primary PBT samples and corresponding PDT cultures.The alternative text for this image may have been generated using AI.

a Comparison of morphological features and expression markers between primary PBT samples and their respective PDT cultures. Hematoxylin and Eosin (H&E) staining and immunohistochemistry analysis are shown. PDTs were harvested for analysis - LGG_12 at day 15, HGG_05 at day 11, and BCOR_02 at day 18 of culture. Markers: GFAP (green): glial fibrillary acidic protein; BRAFV600E: marker for the hotspot mutation; H3K27M: marker for the mutated form of H3 protein. Scale bar: 100 μm (b). Confocal microscopy images of PDTs derived from pediatric low-grade gliomas (LGG_04), an ependymoma (EPM), a medulloblastoma (MB_01), pediatric high-grade gliomas (HGG_02, HGG_04) and a meningioma (MNG), highlighting the expression of lineage/ tumor-specific markers: GFAP (green, glial fibrillary acidic protein), SYP (red, synaptophysin), and MUC-1 (brown, mucin 1). Nuclei are stained in blue (HOESCHT). All images were acquired using the OperaPhenix Plus system. Scale bar: 100 μm. PBT pediatric brain tumor, PDT patient-derived tumoroid.

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