Fig. 2: Transcriptomic analysis of TCMSW620-treated THP-1 macrophages.
A Heatmap of k-means clustering of variably expressed genes in EV/THP-1, S5/THP-1, and S14/THP-1 macrophages with or without TCMSW620 treatment (n = 16,918, k = 6). Genes were grouped into six clusters on the basis of expression similarity. B GO enrichment analysis of genes in each cluster using DAVID, showing top enriched GO terms with a FDR <0.01. C A Heatmap of gene expression levels of SPP1+ and C1QC+ macrophage signature in mock- and TCMSW620-treated EV/THP-1, S5/THP-1, and S14/THP-1 macrophages. D RT-qPCR analysis of mRNA levels of indicated genes in mock- and TCMSW620-treated THP-1 macrophages. Data were normalized with HPRT1 and presented as mean ± SD from three independent experiments. Significance was determined by two-way ANOVA with Tukey’s multiple comparison post hoc test. *P < 0.05; **P < 0.01; ****P < 0.0001. E GSEA showing upregulated genes related to angiogenesis, vasculature development, epithelial development, and positive regulation of cell migration in TCMSW620-treated S14/THP-1 macrophages compared to TCMSW620-treated S5/THP-1 macrophages.
